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For milk samples, pour an agar Manage, pour a dilution h2o Regulate and pipet h2o for the pipet control. Include agar for the latter two for every series of samples. Increase agar promptly to petri dishes when sample diluent is made up of hygroscopic supplies, e.g., flour and starch. Pour agar and dilution h2o control plates for each number of samples. Promptly blend sample dilutions and agar medium carefully and uniformly by alternate rotation and back-and-forth motion of plates on flat amount floor. Let agar solidify. Invert solidified petri dishes, and incubate immediately for 48 ± two h at 35°C. Never stack plates when pouring agar or when agar is solidifying.

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